P149 Malaria genome studies using a full-length-enriched cDNA library
نویسندگان
چکیده
منابع مشابه
FULL-malaria: a database for a full-length enriched cDNA library from human malaria parasite, Plasmodium falciparum
FULL-malaria is a database for a full-length-enriched cDNA library from the human malaria parasite Plasmodium falciparum (http://133.11. 149.55/). Because of its medical importance, this organism is the first target for genome sequencing of a eukaryotic pathogen; the sequences of two of its 14 chromosomes have already been determined. However, for the full exploitation of this rapidly accumulat...
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A large collection of full-length cDNAs is essential for the correct annotation of genomic sequences and for the functional analysis of genes and their products. We obtained a total of 39,936 soybean cDNA clones (GMFL01 and GMFL02 clone sets) in a full-length-enriched cDNA library which was constructed from soybean plants that were grown under various developmental and environmental conditions....
متن کاملAssessment of Redundancy and Full-Length Rate of Full-Length Enriched cDNA Libraries
Collection of full-length genes requires libraries with full-length cDNA insert, large-scale sequencing, library assessment, and high-speed sequence clustering. Here we focus on computational methods, such as newly developed computer programs, since our experimental methods had been published previously. Our purpose is the collection of full-length cDNAs, therefore the proportion of full-length...
متن کاملPreparation of a differentially expressed, full-length cDNA expression library by RecA-mediated triple-strand formation with subtractively enriched cDNA fragments.
We have developed a fast and general method to obtain an enriched, full-length cDNA expression library with subtractively enriched cDNA fragments. The procedure relies on RecA-mediated triple-helix formation of single-stranded cDNA fragments with a double-stranded cDNA plasmid library. The complexes were then captured from the solutions using the digoxigenin-antidigoxigenin paramagnetic beads f...
متن کاملVector-capping: a simple method for preparing a high-quality full-length cDNA library.
Full-length cDNAs play an essential role in identifying genes and determining their promoter regions. Here we describe a simple method for constructing a full-length cDNA library, which has the following advantages: (i) it consists of only three steps including direct ligation between a vector and a cDNA strand using T4 RNA ligase, (ii) it contains neither a PCR process generating mutations nor...
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ژورنال
عنوان ژورنال: Medical Entomology and Zoology
سال: 2001
ISSN: 0424-7086,2185-5609
DOI: 10.7601/mez.52.115_1